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A majority of modern optical microscopy techniques require the use of a digital camera. By working with digital devices, researchers can observe specimens on a screen in real time, or acquire and store images and quantifiable data. A huge range of applications from basic brightfield imaging through to advanced super-resolution techniques all require cameras. The performance and variety of available digital camera technologies has increased considerably, offering researchers a broad spectrum of detectors to address their scientific needs. The choice in digital imaging sensors can have a substantial impact on image characteristics, so it is important to have a basic understanding of how they work and differ from one another. Here we introduce the basic principles behind digital camera technologies commonly encountered in scientific imaging.
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